CRISPRdirect — Rational design of CRISPR/Cas target.  Help

or Paste a nucleotide sequence: ?

or upload sequence file: ?

PAM sequence requirement: (e.g. NGG, NRG, NNGRRT, NG) ?

Specificity check: ?

Whats' new:

This page is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).

If you use CRISPRdirect in your work, please cite:
Naito Y, Hino K, Bono H, Ui-Tei K. (2015)
CRISPRdirect: software for designing CRISPR/Cas guide RNA with reduced off-target sites.
Bioinformatics, 31, 1120-1123. [Full Text]