PAM sequence requirement

An arbitrary 3 nt sequence using IUB codes can be specified in (5).

Example:

IUB codes for nucleic acid bases
CodeBaseComment
G G Guanine
A A Adenine
T T Thymine
C C Cytosine
R A, G puRines
Y C, T pYrimidines
M A, C aMino
K G, T Keto
S C, G Strong
W A, T Weak
H A, C, T not G
B C, G, T not A
V A, C, G not T
D A, G, T not C
N A, C, G, T any