CRISPRdirect — Rational design of CRISPR/Cas target. 

0 mismatch/gap   |   ≤1 mismatch/gap   |   ≤2 mismatches/gaps   |   more:

Search for: both strand plus strand minus strand

2025-06-21 20:51:33, GGGenome : Mouse genome, GRCm39/mm39 (Jun, 2020)

Summary:

Results:

Matches are highlighted with blue background. Mismatches and indels are marked in red.

chr4:155909184-155909198
AAGACTAGGGTTCCTAGATTCAGAACTATT155909184ACCTGAACCCCGTGGACAGTAGCATAGATGAGTTGAGTAAGCTTA
chr7:19718408-19718422
GAGCAATCCTGGATAGAAAATGGCAATCAT19718408ACCTGAACCCCGGGGGTCAGAGCACTCTTCTTTTTTTCTTTTATG
chr8:125275588-125275602
TCAGGCTGACCTGGGCTCAGTGAGTCCCCA125275588ACCTGAACCCCGAGGGCACCTTTACTGCCCTCCCCGCTCAGCACA
chr11:102106580-102106594
AGGAACAGGCAGGGAAAGCCCCGGCTGCAA102106580ACCTGAACCCCGTGGGACTTCCCAACATCCAGAGCATCCTTCCTG
chr3:157998343-157998357
TATTCATGAAAAGGTTAGTAGAACGAATTC157998343CCCCGGGGTTCAGGTTTTTCACTAGGCCCAGAGGAATAGCATAAT
chr15:75619390-75619404
CCACCGCGTCCTCCGGCGTCAGGTTGAGCG75619390CCTCGGGGTTCAGGTGGTGCTGGTACCCGCTCATCCAGTACAGAT
chr17:51883864-51883878
TGAGGGCTGAGAGTATATAAGAGTGAGAGG51883864CCCCGGGGTTCAGGTGAGAAAGATGAGAAAAAGTTGAAGCGAGAG

Data Export:

Maximum 100000 results can be retrieved in various formats shown below:

Debug Info:

Redirect URI : http://crispr.dbcls.jp/detail/en/mm39/ACCTGAACCCCGNGG
lang : en | db : mm39 | k : 0 | strand : | nogap : | query_string : ACCTGAACCCCGNGG | format : html | download : | debug : 

0.006 | 0.006 | search_start;
0.078 | 0.072 | search_plus_done; http://172.18.8.138:28851/match?q=ACCTGAACCCCGNGG&k=0&no_gap=0&offset=0&limit=200
0.082 | 0.003 | search_minus_done; http://172.18.8.138:28851/match?q=CCNCGGGGTTCAGGT&k=0&no_gap=0&offset=0&limit=196
0.082 | 0.001 | cgi_end;

CRISPRdirect | DBCLS
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If you use CRISPRdirect in your work, please cite:
Naito Y, Hino K, Bono H, Ui-Tei K. (2015)
CRISPRdirect: software for designing CRISPR/Cas guide RNA with reduced off-target sites.
Bioinformatics, 31, 1120-1123. [Full Text]