CRISPRdirect — Rational design of CRISPR/Cas target.

gRNA sequence calculator

Input:

Target sequence + PAM:

sgRNA 3′ sequence:

Output:

sgRNA sequence (read only):

RNA→DNA (U→T)

CRISPRdirect | DBCLS
This page is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).

If you use CRISPRdirect in your work, please cite:
Naito Y, Hino K, Bono H, Ui-Tei K. (2015)
CRISPRdirect: software for designing CRISPR/Cas guide RNA with reduced off-target sites.
Bioinformatics, 31, 1120-1123. [Full Text]