CRISPRdirect — Rational design of CRISPR/Cas target. 

0 mismatch/gap   |   ≤1 mismatch/gap   |   ≤2 mismatches/gaps   |   more:

Search for: both strand plus strand minus strand

2025-06-15 03:25:03, GGGenome : Mouse genome, GRCm39/mm39 (Jun, 2020)

Summary:

Results:

Matches are highlighted with blue background. Mismatches and indels are marked in red.

chr4:84103786-84103807
ATTGGTTCCCATAAACTCAGATGCCAAATG84103786TCAGGCC-ATGTCACATTCTAGGGCCTTCAAATTCCAAGCCCCAGTGCTCAGG
chr5:146336828-146336851
CAGAAGGGTGCCAAACAGTTCTGTGTGCTC146336828TCAGCCCAATGTCACATTTGTTGGGATTTGTACAGTATCAAAATGAGGAGATTA
chr1:4240520-4240542
TTGGTCCAGTGACACCTTCATATCCATACA4240520CCCACAATGTGACATTGGCCTGAGTACCTGTGTTTCCAGTCACCACTAAGACC
chr7:117106429-117106450
TTATAGCAGCCTTCTATCAGAGTGCCAAGA117106429CCT-CAATGTGACATTGGCCAGAACCACCTTGAACTTGCTGAGGTGTGATCAG
chr12:83263909-83263931
AGTAATGTTATGGCTTTCTCTGCCTCCACC83263909CCCACAATGTGACAATGGCCTAACATATGGGTTGAATTTCTACTCTTCCATCT

Data Export:

Maximum 100000 results can be retrieved in various formats shown below:

Debug Info:

Redirect URI : http://crispr.dbcls.jp/detail/en/mm39/2/TCAGGCCAATGTCACATTGTNGG
lang : en | db : mm39 | k : 2 | strand : | nogap : | query_string : TCAGGCCAATGTCACATTGTNGG | format : html | download : | debug : 

0.007 | 0.007 | search_start;
0.189 | 0.183 | search_plus_done; http://172.18.8.138:28851/match?q=TCAGGCCAATGTCACATTGTNGG&k=2&no_gap=0&offset=0&limit=200
0.227 | 0.038 | search_minus_done; http://172.18.8.138:28851/match?q=CCNACAATGTGACATTGGCCTGA&k=2&no_gap=0&offset=0&limit=198
0.228 | 0.001 | cgi_end;

CRISPRdirect | DBCLS
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If you use CRISPRdirect in your work, please cite:
Naito Y, Hino K, Bono H, Ui-Tei K. (2015)
CRISPRdirect: software for designing CRISPR/Cas guide RNA with reduced off-target sites.
Bioinformatics, 31, 1120-1123. [Full Text]